ArtNr |
PRS-XR-9940-2mg |
Hersteller |
ProSci
|
Menge |
2 mg |
Kategorie |
|
Typ |
Antibody Secondary |
Format |
Lyophilized |
Applikationen |
ELISA |
Specific against |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Lieferbar |
|
Conjugate |
Biotin |
Applications |
Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2, 2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50, 000 to 1:500, 000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user). |
Applications |
Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2, 2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50, 000 to 1:500, 000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user). |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin |
Storage Conditions |
Biotin Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 ˚ C prior to restoration. For extended storage mix product with 50% glycerol and then aliquot contents and freeze at -20 ˚ C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ˚ C as an undiluted liquid. Dilute only prior to immediate use. |
Disclaimer |
This product is for research use only. |
Immunogen |
Rabbit IgG - whole molecule |
Specificity |
Minimum Cross reactivity to Human Serum Proteins |
Purification |
Biotin Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins. |
Isotype |
IgG |
By Conjugate |
Biotin |
By Species |
Anti-Rabbit |
Target Species |
Reacts with the heavy chain of rabbit IgG. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.