ArtNr |
PRS-XP-5242-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Lyophilized |
Applikationen |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CKb7, PARC, AMAC1, DCCK1, MIP-4, AMAC-1, DC-CK1, SCYA18, MIP4, C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Chemokines & Cytokines, Antibody Pairs, Functional Assays |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-4 (human Macrophage Inflammatory Protein-4). |
Applications |
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIPu4 (100.0 ng/mL), a concentration of 8.0 - 10.0 μ g/mL of this antibody is required.
ELISA:
To detect hMIP-4 by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-4.
Sandwich:
To detect hMIP-4 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-4 (XP-5242Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4.
Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Purification |
Anti-hMIP-4 specific antibody was purified by affinity chromatography employing immobilized hMIP-4 matrix. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Storage Conditions |
MIP-4 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CCL18 |
Accession # |
P55774 |
Protein Gi # |
2493666 |
Ncbi Gene Id # |
6362 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
CCL18 |
Ncbi Official Full Name |
chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P55774 |
1st Image Caption |
To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
2nd Image Caption |
To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
3rd Image Caption |
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 buffer is recommended. Optimal concentrations and conditions may vary. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.