IL-1a Antibody (biotin)

Immunology, Chemokines & Cytokines, Antibody Pairs

ELISA:

Sandwich:
To detect mIL-1α by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-1α (XP-5175) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1α .

Western Blot:
To detect mIL-1- α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1- α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

IL-1a antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

None

P01582

16175

Il1a

Mus musculus

To detect mIL-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine IL-1-alpha (XP-5175) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-1-alpha.