ArtNr |
PRS-PM-5179-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
WB, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
PD-1 Antibody [12A7D7] : PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis |
Immunogen |
A ~150 amino acid recombinant protein from near the amino terminus of mouse PD-1. |
Applications |
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 95-115 - PD-1 Recombinant Protein |
Purification |
PD-1 Monoclonal Antibody is Protein A purified. |
Clonality |
Monoclonal |
Clone |
12A7D7 |
Isotype |
IgG1 |
Conjugate |
Unconjugated |
Buffer |
PD-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
PD-1 monoclonal antibody can be stored at -20˚ C, stable for one year. |
Related Products |
Cat. No. 95-115 - PD-1 Recombinant Protein |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PDCD1 |
Accession # |
EDL39922 |
Protein Gi # |
148707975 |
Ncbi Gene Id # |
5133 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PDCD1 |
Ncbi Official Full Name |
programmed cell death 1 |
Ncbi Organism |
mus musculus |
Swissprot # |
Q15116 |
Background |
PD-1 Monoclonal Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
Background References 1 |
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. |
Background References 2 |
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. |
Background References 3 |
Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8. |
Background References 4 |
Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51. |
1st Image Caption |
Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunohistochemistry of PD-1 in mouse thymus tissue with PD-1 antibody at 5 μ g/mL. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.