ORAI3 Antibody [2H2G9]

blue ice or RT

A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.

Cat. No. 1466 - Rat Spleen Tissue Lysate

ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

2H2G9

Unconjugated

1 mg/mL

Cat. No. 4215P ORAI3 Peptide

None

Q9BRQ5

93129

ORAI3

homo sapiens

ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.

Immunohistochemistry of ORAI3 in rat spleen tissue with ORAI3 antibody at 2.5 μ g/mL.

Immunofluorescence of ORAI3 in human spleen tissue with ORAI3 antibody at 5 μ g/ml.

Green: ORAI3 antibody (PM-4911)
Red: Phylloidin staining
Blue: DAPI staining