CD276 Antibody

Cell Cycle, Cancer, Immunology

CD276 antibody can be used for Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for Immunohistochemistry at 2 μ g/mL. For Immunoflorescence start at 20 μ g/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Cat. No. 1306 - Human Spleen Tissue Lysate

Predicted: 35, 43, 57 kDa

Observed: 59 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 8689P - CD276 Peptide

None

NP_001019907

80381

CD276

Homo sapiens

CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.

Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7.

Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe?. Int J Cancer 2014; 134:2764-71.

Immunohistochemistry of CD276 in human spleen tissue with CD276 antibody at 2 μ g/ml.