CD80 Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Rabbit: (71%)

CD80 antibody can be used for Western blot at 1 - 2 μ g/mL. Antibody can also be used for Immunohistochemistry at 2 μ g/mL. For Immunoflorescence start at 20 μ g/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Predicted: 32 kDa

Observed: 50 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 8679P - CD80 Peptide

None

NP_005182

941

CD80

Homo sapiens

CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).

Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 2 μ g/ml.