ArtNr |
PRS-8055-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Stem Cell |
Immunogen |
HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.
The immunogen is located within the first 50 amino acids of HES5. |
Applications |
HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. |
Specificity |
HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms. |
Positive Control 1 |
Cat. No. 1207 - Raji Cell Lysate |
Positive Control 2 |
Cat. No. 10-901 - Human Spleen Tissue Slide |
Predicted Molecular Weight |
Predicted: 18 kDa
Observed: 17 kDa |
Purification |
HES5 antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
HES5 antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
HES5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 8055P – HES5 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HES5 |
Accession # |
NP_001010926 |
Protein Gi # |
58219048 |
Ncbi Gene Id # |
388585 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HES5 |
Ncbi Official Full Name |
hes family bHLH transcription factor |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q5TA89 |
Background |
HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3). |
Background References 1 |
Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34. |
Background References 2 |
Karlsson C, Jonsson M, Asp J, et al. Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 2007; 327:539-51. |
Background References 3 |
Sancho R, Blake SM, Tendeng C, et al. Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions. PLoS Biol. 2013; 11:e1001586. |
1st Image Caption |
Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μ g/ml (A) the absence and (B) the presence of blocking peptide. |
2nd Image Caption |
Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μ g/ml. |
3rd Image Caption |
Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μ g/ml. |
4th Image Caption |
Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 μ g/mL. |
5th Image Caption |
Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 μ g/mL.
Green: HES5 Antibody (8055) Blue: DAPI staining |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.