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PIK3R4 Antibody

PIK3R4 Antibody

ArtNr	PRS-7949-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Oncology Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status Adenocarcinoma Mesothelioma Immunohistochemistry Western Blot ELISA Immunofluorescence Autophagy Cardiovascular Research Proliferation Myocardial Infarction Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Phosphoinositide-3-kinase regulatory subunit 4, p150, Phosphoinositide 3-kinase adaptor protein, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, VPS15
Lieferbar
Shipping	blue ice or RT
By Research Area	Autophagy
Immunogen	PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4. The immunogen is located within amino acids 1290 - 1340 of PIK3R4.
Applications	PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	PIK3R4 antibody is human, mouse and rat reactive.
Positive Control 1	Cat. No. 1315 - Human Tonsil Tissue Lysate
Predicted Molecular Weight	Predicted: 149 kDa Observed: 140 kDa
Purification	PIK3R4 antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	PIK3R4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7949P – PIK3R4 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PIK3R4
Accession #	NP_055417
Protein Gi #	23943912
Ncbi Gene Id #	30849
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PIK3R4
Ncbi Official Full Name	phosphoinositide 3-kinase regulatory subunit 4
Ncbi Organism	homo sapiens
Swissprot #	Q99570
Background	PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1, 2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).
Background References 1	Herman PK, Stack JH, and Emr SD. A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery. EMBO J. 1991; 10:4049-60.
Background References 2	Stasyk OV, van der Klei IJ, Bellu AR, et al. A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy. Curr. Genet. 1999; 36:262-9.
Background References 3	Kihara A, Noda T, Ishihara N, et al. Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J. Cell Biol. 2001; 152:519-30.
Background References 4	Stack JH, DeWald DB, Takegawa K, et al. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J. Cell Biol. 1995; 129:321-34.
1st Image Caption	Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 μ g/ml.
2nd Image Caption	Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 μ g/ml.
3rd Image Caption	Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μ g/ml.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.1 mg

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