ArtNr |
PRS-79-384-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IHC |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
p65, NFKB3, NACP, PARK1, RELA, TF65, Transcription factor p65, p65 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cancer |
Immunogen |
NFkB-p65 (Ab-311) antibody was raised against a peptide sequence around aa.309~313 (F-K-S-I-M) derived from Human NFκ B-p65. |
Applications |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
Specificity |
This antibody detects endogenous level of total NFκ B-p65 protein. |
Predicted Molecular Weight |
65 kDa |
Purification |
Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration |
1 mg/mL |
Storage Conditions |
Store antibody at -20˚ C for up to one year. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RELA |
Accession # |
NP_001138610.1 |
Protein Gi # |
223468681 |
Ncbi Gene Id # |
5970 |
Ncbi Official Symbol |
RELA |
Ncbi Official Full Name |
v-rel avian reticuloendotheliosis viral oncogene homolog A |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q04206 |
Background |
NF-κ -B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ -B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κ -B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-κ -B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κ -B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κ -B complex which translocates to the nucleus. NF-κ -B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-κ -B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-κ -B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-κ -B complex |
Background References 1 |
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289. |
1st Image Caption |
Western blot analysis of lysed extracts from HeLa and HL60 cells using NFκ B-p65 (Ab-311). |
2nd Image Caption |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκ B-p65 (Ab-311). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.