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NFkB p65 (Ab 311) Antibody

ArtNr PRS-79-384-0.1mg
Hersteller ProSci
Menge 0.1 mg
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IHC
Specific against Human
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias p65, NFKB3, NACP, PARK1, RELA, TF65, Transcription factor p65, p65
Lieferbar
Shipping
blue ice or RT
By Research Area
Apoptosis, Cancer
Immunogen
NFkB-p65 (Ab-311) antibody was raised against a peptide sequence around aa.309~313 (F-K-S-I-M) derived from Human NFκ B-p65.
Applications
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity
This antibody detects endogenous level of total NFκ B-p65 protein.
Predicted Molecular Weight
65 kDa
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/mL
Storage Conditions
Store antibody at -20˚ C for up to one year.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
RELA
Accession #
NP_001138610.1
Protein Gi #
223468681
Ncbi Gene Id #
5970
Ncbi Official Symbol
RELA
Ncbi Official Full Name
v-rel avian reticuloendotheliosis viral oncogene homolog A
Ncbi Organism
Homo sapiens
Swissprot #
Q04206
Background
NF-κ -B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ -B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κ -B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-κ -B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κ -B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κ -B complex which translocates to the nucleus. NF-κ -B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-κ -B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-κ -B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-κ -B complex
Background References 1
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.
1st Image Caption
Western blot analysis of lysed extracts from HeLa and HL60 cells using NFκ B-p65 (Ab-311).
2nd Image Caption
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκ B-p65 (Ab-311).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
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