ArtNr |
PRS-7809-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Vinculin Antibody: MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Homology |
Predicted species reactivity based on immunogen sequence: Pig: (100%) |
Immunogen |
Anti-Vinculin antibody (7809) was raised against a peptide corresponding to 23 amino acids near the center of human Vinculin. < |
Applications |
WB: 0.5-2 μ g/mL; ICC: 5 μ g/mL; IF: 20 μ g/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist. |
Positive Control 1 |
Cat. No. 1202 - A431 Cell Lysate |
Positive Control 2 |
Cat. No. 1201 - HeLa Cell Lysate |
Positive Control 3 |
Cat. No. 1282 - 3T3/NIH Cell Lysate |
Predicted Molecular Weight |
Predicted: 124kD
Observed: 124kD |
Validation |
Independent Antibody Validation in Cell lines (Figure 2) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain. Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. |
Isoforms |
Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD). 7809 can detect human, mouse and rat. |
Purification |
Vinculin antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
Vinculin antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
Vinculin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 7809P – Vinculin Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
VCL |
Accession # |
NP_003364 |
Protein Gi # |
4507877 |
Ncbi Gene Id # |
7414 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
VCL |
Ncbi Official Full Name |
vinculin |
Ncbi Organism |
homo sapiens |
Swissprot # |
P18206 |
Background |
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2, 3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5). |
Background References 1 |
Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525. |
Background References 2 |
Gilmore et al. J. Cell Sci. 1992; 103:719-31. |
Background References 3 |
Deakin et al. PLoS One 2012; 7:e37990. |
Background References 4 |
Goldmann et al. Cell Biol. Int. 2013; Feb 1. |
Citation 1 |
Wang et al. AMPK is involved in the differential neonatal performance of chicks hatching at different time. Gen Comp Endocrinol. 2016; 228:53-59. PMID: 26873631 |
1st Image Caption |
Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: Vinculin 7489 (2 μ g/mL), Vinculin 7807 (1 μ g/mL), Vinculin 7809 (1 µg/mL), and beta-actin 3779 (1.5 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
2nd Image Caption |
Figure 2 Western Blot Validation in Human, Mouse and Rat Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: Vinculin 7809 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
3rd Image Caption |
Figure 3 Western Blot Validation in PC3 Cell Lysate Loading: 15 μ g of lysates per lane.Antibodies: Vinculin 7809 (Lane 1: 0.5 μ g/mL and Lane 2: 1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
4th Image Caption |
Figure 4 Immunocytochemistry Validation of Vinculin in Jurkat Cells Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (7809) at 5 μ g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
5th Image Caption |
Figure 5 Immunofluorescence Validation of Vinculin in Jurkat Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with 7809 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.