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ARF6 Antibody

ARF6 Antibody

ArtNr	PRS-7699-0.02mg
Hersteller	ProSci
Menge	0.02 mg
Kategorie	Neuroscience Protein Aggregation, Clearance & Misfolding Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ARF6 Antibody: ADP-ribosylation factor 6
Lieferbar
Shipping	blue ice or RT
By Research Area	Signal Transduction
Homology	Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Immunogen	ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6. The immunogen is located within the last 50 amino acids of ARF6.
Applications	ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	ARF6 antibody is human, mouse and rat reactive.
Positive Control 1	Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2	Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight	Predicted: 19 kDa Observed: 18 kDa
Purification	ARF6 antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	ARF6 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	ARF6 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7699P – ARF6 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ARF6
Accession #	NP_001654
Protein Gi #	4502211
Ncbi Gene Id #	382
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ARF6
Ncbi Official Full Name	ADP-ribosylation factor 6
Ncbi Organism	homo sapiens
Swissprot #	P62330
Background	The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1). The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D (2). The product of this gene is localized to the plasma membrane (3), and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling (4).
Background References 1	Graves RA, Tontonoz P, and Spiegelman BM. Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Mol. Cell Biol. 1992; 12:1202-8.
Background References 2	Moss J and Vaughan M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem. 1999; 193:153-7.
Background References 3	Cavenagh MM, Whitney JA, Carroll K, et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J. Biol. Chem. 1996; 271:21767-74.
Background References 4	Schweitzer JK, Sedgewick AE, and D’Souza-Schorey C. ARF6-mediated endocytic recycling impacts cell movement, cell division and lipid homestasis. Semin. Cell Dev. Biol. 2011; 22:39-47.
1st Image Caption	Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 μ g/ml.
2nd Image Caption	Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 μ g/mL.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.02 mg

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