ArtNr |
PRS-7699-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
ARF6 Antibody: ADP-ribosylation factor 6 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%) |
Immunogen |
ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6.
The immunogen is located within the last 50 amino acids of ARF6. |
Applications |
ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
ARF6 antibody is human, mouse and rat reactive. |
Positive Control 1 |
Cat. No. 1464 - Rat Liver Tissue Lysate |
Positive Control 2 |
Cat. No. 10-401 - Human Kidney Tissue Slide |
Predicted Molecular Weight |
Predicted: 19 kDa
Observed: 18 kDa |
Purification |
ARF6 antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
ARF6 antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
ARF6 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 7699P – ARF6 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ARF6 |
Accession # |
NP_001654 |
Protein Gi # |
4502211 |
Ncbi Gene Id # |
382 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ARF6 |
Ncbi Official Full Name |
ADP-ribosylation factor 6 |
Ncbi Organism |
homo sapiens |
Swissprot # |
P62330 |
Background |
The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1). The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D (2). The product of this gene is localized to the plasma membrane (3), and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling (4). |
Background References 1 |
Graves RA, Tontonoz P, and Spiegelman BM. Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Mol. Cell Biol. 1992; 12:1202-8. |
Background References 2 |
Moss J and Vaughan M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem. 1999; 193:153-7. |
Background References 3 |
Cavenagh MM, Whitney JA, Carroll K, et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J. Biol. Chem. 1996; 271:21767-74. |
Background References 4 |
Schweitzer JK, Sedgewick AE, and D’Souza-Schorey C. ARF6-mediated endocytic recycling impacts cell movement, cell division and lipid homestasis. Semin. Cell Dev. Biol. 2011; 22:39-47. |
1st Image Caption |
Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 μ g/ml. |
2nd Image Caption |
Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.