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MAP1LC3B Antibody

MAP1LC3B Antibody

ArtNr	PRS-7581-0.02mg
Hersteller	ProSci
Menge	0.02 mg
Kategorie	Oncology Neuroscience Neural Lineage Markers Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Testicular & Prostate Cancer Oligodendrocyte Markers Astroglia Markers
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy-related protein LC3 B
Lieferbar
Shipping	blue ice or RT
By Research Area	Autophagy
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen	MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B. The immunogen is located within the first 50 amino acids of MAP1LC3B.
Applications	MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C
Positive Control 1	Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2	Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight	Predicted: 14 kDa Observed: 14 kDa
Purification	MAP1LC3B antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	MAP1LC3B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7581P – MAP1LC3B Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	MAP1LC3B
Accession #	NP_073729
Protein Gi #	12383056
Ncbi Gene Id #	81631
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	MAP1LC3B
Ncbi Official Full Name	microtubule-associated protein 1 light chain 3 beta
Ncbi Organism	homo sapiens
Swissprot #	Q9GZQ8
Background	Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).
Background References 1	Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
Background References 2	Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85.
Background References 3	Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012; 8:1724-40.
Background References 4	Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8.
1st Image Caption	Western blot analysis of MAP1LC3B in human brain tissue lysate with MAP1LC3B antibody at 1 μ g/ml.
2nd Image Caption	Immunohistochemistry of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 20 μ g/mL.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.02 mg

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