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MTERFD1 Antibody

MTERFD1 Antibody

ArtNr	PRS-7281-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ICC, ELISA
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	MTERFD1 Antibody: CGI-12, mTERF3, mTERF domain-containing protein 1, mitochondrial, Mitochondrial transcription termination factor 3
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Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle
Homology	Predicted species reactivity based on immunogen sequence: Rat: (88%), Chicken: (88%)
Immunogen	MTERFD1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MTERFD1 . The immunogen is located within the last 50 amino acids of MTERFD1.
Applications	MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 μ g/mL. Antibody can also be used for Immunocytochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members.
Positive Control 1	Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2	Cat. No. 17-201 - 3T3/BALB Cell Slide
Predicted Molecular Weight	Predicted: 46 kDa Observed: 42 kDa
Purification	MTERFD1 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	MTERFD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	MTERFD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7281P – MTERFD1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	MTERFD1
Accession #	NP_057026
Protein Gi #	34147676
Ncbi Gene Id #	51001
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	MTERFD1
Ncbi Official Full Name	MTERF domain containing 1
Ncbi Organism	homo sapiens
Swissprot #	Q96E29
Background	MTERFD1 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD1 is composed of three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It binds promoter DNA and acts as a mitochondrial transcription regulator. It has been suggested that only the phosphorylated form of MTERFD1 has transcription termination activity. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.
Background References 1	Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79.
Background References 2	Hyvarinen AK, Pohjoismaki JL, Holt IJ, et al. Overexpression of MTERFD1 or MTERFD3 impairs the completion of mitochondrial DNA replication. Mol. Biol. Rep. 2011; 38:1321-8.
Background References 3	Prieto-Martin A, Montoya J, Martinez-Azorin F, et al. Phosphorylation of rat mitochondrial transcription termination factor (mTERF) is required for transcription termination but not for binding to DNA. Nucleic Acids Res. 2004; 32:2059-68.
Background References 4	Park CB, Asin-Cayuela J, Camara Y, et al. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 2007; 130:273-85.
1st Image Caption	Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μ g/mL.

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Menge: 0.1 mg

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