NELF Antibody

Stem Cell

NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Predicted: 58 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 7225P – NELF Peptide

None

NP_056352

26012

NELF

homo sapiens

NELF Antibody: NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH), a disorder resulting in impaired pubertal maturation and reproductive function.

Yung TM, Narita T, Komori T, et al. Cellular dynamics of the negative transcription elongation factor NELF. Exp. Cell Res. 2009; 315:1693-705.

Miura K, Acierno JS and Seminara SB. Characterization of the human nasal embryonic LHRH factor gene, NELF, and a mutation screening among 65 patients with idiopathic hypogonadotropic hypogonadism (IHH). J. Hum. Genet. 2004; 49: 265-8.

Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 μ g/ml.

Immunohistochemistry of NELF in mouse brain tissue with NELF Antibody at 5 μ g/mL.