« Zurück
Home /
Fat Free Antibody

Fat Free Antibody

Hersteller	ProSci
Kategorie	Neuroscience Neural Signaling Antibodies Western Blot ELISA Primary Antibodies
Typ	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Format	Liquid
Applikationen	WB, ELISA
Menge	0.02 mg
Host	Rabbit
ArtNr	PRS-6979-0.02mg
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Alias	Fat Free Antibody: FFR, ANG2, ANG3, C11orf2, C11orf3, FFR, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein
Shipping	blue ice or RT
By Research Area	Homeostasis
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (82%)
Immunogen	Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free. The immunogen is located within amino acids 620 - 670 of Fat Free.
Applications	Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μ g/mL. Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity	At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.
Positive Control 1	Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight	86 kDa
Purification	Fat Free Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	Fat Free antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6979P – Fat-free Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	C11orf2
Accession #	NP_037397
Protein Gi #	8393009
Ncbi Gene Id #	738
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	C11orf2
Ncbi Official Full Name	chromosome 11 open reading frame 2
Ncbi Organism	homo sapiens
Swissprot #	Q9UID3
Background	Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.
Background References 1	Perez-Victoria FJ, Schindler C, Magadan JG, et al. Ang2/Fat Free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol. Biol. Cell 2010; 21:3386-95
Background References 2	Ho SY, Lorent K, Pack M, et al. Zebrafish Fat Free is required for intestinal lipid absorption and Golgi apparatus structure. Cell Metab. 2006; 3:289-300.
Background References 3	Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell 2008; 19:2350-62
Background References 4	Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.
1st Image Caption	Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μ g/mL.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

Produkdatenblatt anfordern

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 0.02 mg

lieferbar

Lieferung vsl. bis 02.08.2024

Express-Lieferung: 11 Tage sparen

Vergleichen

Auf den Wunschzettel

Weiterempfehlen per Mail