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CLEC7A Antibody

CLEC7A Antibody

ArtNr	PRS-6833-0.02mg
Hersteller	ProSci
Menge	0.02 mg
Kategorie	Oncology Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Colorectal Cancer
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CLEC7A Antibody: BGR, CANDF4, DECTIN1, CLECSF12, BGR, UNQ539/PRO1082, C-type lectin domain family 7 member A, Beta-glucan receptor, DC-associated C-type lectin 1
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Shipping	blue ice or RT
By Research Area	Innate Immunity
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen	CLEC7A antibody was raised against a 17 amino acid synthetic peptide near the center of human CLEC7A. The immunogen is located within amino acids 70 - 120 of CLEC7A.
Applications	CLEC7A antibody can be used for detection of CLEC7A by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	Multiple isoforms of CLEC7A are known to exist.
Positive Control 1	Cat. No. 1466 - Rat Spleen Tissue Lysate
Positive Control 2	Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight	Predicted: 27 kDa Observed: 27 kDa
Purification	CLEC7A Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	CLEC7A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	CLEC7A antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6833P – CLEC7A Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CLEC7A
Accession #	NP_922938
Protein Gi #	37675373
Ncbi Gene Id #	64581
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	CLEC7A
Ncbi Official Full Name	C-type lectin domain family 7, member A
Ncbi Organism	Homo sapiens
Swissprot #	Q9BXN2
Background	CLEC7A Antibody: CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1, 3-linked and beta-1, 6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
Background References 1	Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J. Immunol. 2002; 169:3876-82.
Background References 2	Ebner S, Sharon S, and Ben-Tal N. Evolutionary analysis reveals collective properties and specificity in the C-type lectin and lectin-like domain superfamily. Proteins 2003; 53:44-55.
Background References 3	Kerrigan AM and Brown GD. Syk-coupled C-type lectin receptors that mediate cellular activation via single tyrosine based activation motifs. Immunol. Rev. 2010; 234:335-52.
Background References 4	Underhill DM, Rossnagle E, Lowell CA, et al. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 2005; 106:2543-50.
1st Image Caption	Western blot analysis of CLEC7A in rat spleen tissue lysate with CLEC7A antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of CLEC7A in human spleen tissue with CLEC7A antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of CLEC7A in Jurkat cells with CLEC7A antibody at 20 μ g/mL.

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