Vergleich

SPATA3 Antibody

Hersteller ProSci
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse
Format Liquid
Applikationen WB, IF, ELISA, IHC-P
Menge 0.02 mg
Host Rabbit
ArtNr PRS-6551-0.02mg
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Alias SPATA3 Antibody: TSARG1, TSARG1, Spermatogenesis-associated protein 3, Testis and spermatogenesis cell-related protein 1
Shipping
blue ice or RT
By Research Area
Apoptosis
Immunogen
SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3.

The immunogen is located within the last 50 amino acids of SPATA3.
Applications
SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity
At least three isoforms of SPATA3 are known to exist; this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members
Positive Control 1
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2
Cat. No. 1223 - Caco-2 Cell Lysate
Predicted Molecular Weight
Predicted: 21 kDa

Observed: 26 kDa
Purification
SPATA3 Antibody is affinity chromatography purified via peptide column.
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
SPATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL
Storage Conditions
SPATA3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products
Cat. No. 6551P – SPATA3 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
SPATA3
Accession #
NP_620712
Protein Gi #
31712020
Ncbi Gene Id #
130560
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
SPATA3
Ncbi Official Full Name
spermatogenesis associated 3
Ncbi Organism
Homo sapiens
Swissprot #
Q8NHX4
Background
SPATA3 Antibody: The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.
Background References 1
Fu JJ, Lu GX, Li LY, et al. Molecular cloning for testis spermatogenesis cell apoptosis related gene TSARG1 and Mtsarg1 and expression analysis for Mtsarg1 gene. Yi Chuan Xue Bao 2003; 30:25-9.
Background References 2
Yang HM, Liu G, Nie ZY, et al. Molecular cloning of a novel rat gene Tsarg1, a member of the DnaJ/HSP40 protein family. DNA Seq. 2005; 16:166-72.
1st Image Caption
Western blot analysis of SPATA3 in 3T3 cell lysate with SPATA3 antibody at 1 μ g/mL.
2nd Image Caption
Immunohistochemistry of SPATA3 in mouse testis tissue with SPATA3 antibody at 2.5 μ g/mL.
3rd Image Caption
Immunofluorescence of SPATA3 in mouse testis tissue with SPATA3 antibody at 20 μ g/mL.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.02 mg
Lieferbar: In stock
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