Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human, Mouse |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Menge |
0.02 mg |
Host |
Rabbit |
ArtNr |
PRS-6551-0.02mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Alias |
SPATA3 Antibody: TSARG1, TSARG1, Spermatogenesis-associated protein 3, Testis and spermatogenesis cell-related protein 1 |
Shipping |
blue ice or RT |
By Research Area |
Apoptosis |
Immunogen |
SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3.
The immunogen is located within the last 50 amino acids of SPATA3. |
Applications |
SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Specificity |
At least three isoforms of SPATA3 are known to exist; this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members |
Positive Control 1 |
Cat. No. 1224 - Daudi Cell Lysate |
Positive Control 2 |
Cat. No. 1223 - Caco-2 Cell Lysate |
Predicted Molecular Weight |
Predicted: 21 kDa
Observed: 26 kDa |
Purification |
SPATA3 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
SPATA3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
SPATA3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 6551P – SPATA3 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SPATA3 |
Accession # |
NP_620712 |
Protein Gi # |
31712020 |
Ncbi Gene Id # |
130560 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SPATA3 |
Ncbi Official Full Name |
spermatogenesis associated 3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q8NHX4 |
Background |
SPATA3 Antibody: The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells. |
Background References 1 |
Fu JJ, Lu GX, Li LY, et al. Molecular cloning for testis spermatogenesis cell apoptosis related gene TSARG1 and Mtsarg1 and expression analysis for Mtsarg1 gene. Yi Chuan Xue Bao 2003; 30:25-9. |
Background References 2 |
Yang HM, Liu G, Nie ZY, et al. Molecular cloning of a novel rat gene Tsarg1, a member of the DnaJ/HSP40 protein family. DNA Seq. 2005; 16:166-72. |
1st Image Caption |
Western blot analysis of SPATA3 in 3T3 cell lysate with SPATA3 antibody at 1 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of SPATA3 in mouse testis tissue with SPATA3 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of SPATA3 in mouse testis tissue with SPATA3 antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.