ArtNr |
PRS-63-943-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle, Cell Cycle, Obesity, Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Xenopus |
Immunogen |
This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-139 amino acids from the N-terminal region of human HNF4A. |
Applications |
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
53 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HNF4A |
Accession # |
P41235 |
Protein Gi # |
148886624 |
Ncbi Gene Id # |
3172 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HNF4A |
Ncbi Official Full Name |
Hepatocyte nuclear factor 4-alpha |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P41235 |
Background |
HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. |
Background References 1 |
Koh, W., et al. BMC Genomics 11 SUPPL 1, S6 (2010) |
Background References 2 |
Wang, F., et al. Chin. Med. J. 122(20):2477-2482(2009) |
Background References 3 |
Taghavi, S.M., et al. Cardiovasc Diabetol 8, 63 (2009) |
Background References 4 |
Guo, H., et al. Biochem. J. 394 (PT 2), 379-387 (2006) |
1st Image Caption |
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane. |
3rd Image Caption |
Confocal immunofluorescent analysis of HNF4A Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.