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SRC Antibody

SRC Antibody

ArtNr	PRS-63-539-400ul
Hersteller	ProSci
Menge	400 ul
Kategorie	Metabolism Cholesterol & Lipid Metabolism Antibodies Obesity Immunohistochemistry Western Blot Immunofluorescence Enzymes Other Fatty Acid Enzymes Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, IHC-P
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src, SRC, SRC1
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Shipping	blue ice or RT
By Research Area	Cancer, Signal Transduction
Homology	Predicted species reactivity based on immunogen sequence: Mouse, Rat, Zebrafish, Pig, Chicken, Xenopus, B
Immunogen	This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-424 amino acids from human SRC.
Applications	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50
Predicted Molecular Weight	60 kDa
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	SRC
Accession #	P12931
Protein Gi #	125711
Ncbi Gene Id #	6714
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	SRC
Ncbi Official Full Name	Proto-oncogene tyrosine-protein kinase Src
Ncbi Organism	Homo sapiens
Swissprot #	P12931
Background	SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Background References 1	Muller, A.J., et.al., Mol. Cell. Biol. 12 (11), 5087-5093 (1992)
Background References 2	MacAuley, A., et.al., Oncogene(1), 117-124 (1993)
Background References 3	den Hertog, J., et.al., EMBO J. 12 (10), 3789-3798 (1993)
1st Image Caption	Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419) at 1:1000.
2nd Image Caption	Western blot analysis of SRC Antibody (Y419) in HepG2 cell line lysates (35ug/lane)
3rd Image Caption	Formalin-fixed and paraffin-embedded human hepatocarcinoma with SRC Antibody (Y419), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
4th Image Caption	Confocal immunofluorescent analysis of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 400 ul

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Lieferung vsl. bis 06.09.2024

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