Vergleich

HPD Antibody

ArtNr PRS-63-536-400ul
Hersteller ProSci
Menge 400 ul
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, FC, IHC-P
Specific against Human, Mouse
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias 4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD
Lieferbar
Shipping
blue ice or RT
By Research Area
Cell Cycle, Obesity, Signal Transduction
Immunogen
This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human HPD.
Applications
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Predicted Molecular Weight
45 kDa
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
Rabbit Ig
Conjugate
Unconjugated
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration
batch dependent
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
HPD
Accession #
P32754
Protein Gi #
417144
Ncbi Gene Id #
3242
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
HPD
Ncbi Official Full Name
4-hydroxyphenylpyruvate dioxygenase
Ncbi Organism
Homo sapiens
Swissprot #
P32754
Background
HPD is an enzyme in the catabolic pathway of tyrosine. This protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate.
Background References 1
Item, C.B., et.al., Mol. Genet. Metab. 91 (4), 379-383 (2007)
Background References 2
Wistow, G., et.al., Mol. Vis. 8, 171-184 (2002)
1st Image Caption
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
2nd Image Caption
Western blot analysis of HPD Antibody in mouse liver tissue lysates (35ug/lane)
3rd Image Caption
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
4th Image Caption
Flow cytometric analysis of HepG2 cells using HPD Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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