ArtNr |
PRS-63-536-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, FC, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Obesity, Signal Transduction |
Immunogen |
This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human HPD. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
45 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HPD |
Accession # |
P32754 |
Protein Gi # |
417144 |
Ncbi Gene Id # |
3242 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HPD |
Ncbi Official Full Name |
4-hydroxyphenylpyruvate dioxygenase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P32754 |
Background |
HPD is an enzyme in the catabolic pathway of tyrosine. This protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. |
Background References 1 |
Item, C.B., et.al., Mol. Genet. Metab. 91 (4), 379-383 (2007) |
Background References 2 |
Wistow, G., et.al., Mol. Vis. 8, 171-184 (2002) |
1st Image Caption |
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
Western blot analysis of HPD Antibody in mouse liver tissue lysates (35ug/lane) |
3rd Image Caption |
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
4th Image Caption |
Flow cytometric analysis of HepG2 cells using HPD Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.