Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human |
Format |
Liquid |
Applikationen |
WB, IHC-P |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
PRS-63-432-400ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Alias |
Nucleoside diphosphate kinase, mitochondrial, NDK, NDP kinase, mitochondrial, Nucleoside diphosphate kinase D, NDPKD, nm23-H4, NME4, NM23D |
Shipping |
blue ice or RT |
By Research Area |
Obesity, Neuroscience, Signal Transduction |
Immunogen |
This NME4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-187 amino acids from the C-terminal region of human NME4. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 |
Predicted Molecular Weight |
21 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NME4 |
Accession # |
O00746 |
Ncbi Gene Id # |
4833 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
NME4 |
Ncbi Official Full Name |
Nucleoside diphosphate kinase, mitochondrial |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O00746 |
Background |
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). |
Background References 1 |
Milon, L., et al., Hum. Genet. 99(4):550-557 (1997). |
1st Image Caption |
Western blot analysis of hNME4-V173 in HL-60 cell line lysates (35ug/lane) |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.