ArtNr |
PRS-63-295-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IP, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Serine/threonine-protein kinase pim-2, Pim-2h, PIM2 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
This PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-308 amino acids from the C-terminal region of human PIM2. |
Applications |
For WB starting dilution is: 1:1000
For IP starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100 |
Predicted Molecular Weight |
34 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PIM2 |
Accession # |
Q9P1W9 |
Protein Gi # |
20139243 |
Ncbi Gene Id # |
11040 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PIM2 |
Ncbi Official Full Name |
Serine/threonine-protein kinase pim-2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9P1W9 |
Background |
Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively regulated by growth factor depletion. Increased levels of Pim-2 has also been observed in certain cancers. |
Background References 1 |
Yan, B., et al., J. Biol. Chem. 278(46):45358-45367 (2003). |
Background References 2 |
Baytel, D., et al., Biochim. Biophys. Acta 1442 (2-3), 274-285 (1998). |
1st Image Caption |
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
Western blot analysis of anti-PIM2 Antibody in Hela cell line lysates (35ug/lane) |
3rd Image Caption |
Western blot analysis of PIM2 using rabbit polyclonal PIM2 Antibody (D292) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene. |
4th Image Caption |
PIM proteins were immunoprecipitated from MV4; 11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4; 11 extract and used for the same in vitro phosphorylation reaction as a negative control. |
5th Image Caption |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.