ArtNr |
PRS-63-210-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, FC, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450 HLp2, Cytochrome P450-PCN3, CYP3A5 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Obesity, Signal Transduction |
Immunogen |
This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-218 amino acids from the Central region of human CYP3A5. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
57 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CYP3A5 |
Accession # |
P20815 |
Protein Gi # |
117157 |
Ncbi Gene Id # |
1577 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CYP3A5 |
Ncbi Official Full Name |
Cytochrome P450 3A5 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P20815 |
Background |
CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione. |
Background References 1 |
Zencir, S., Z. Naturforsch., C, J. Biosci. 63 (9-10), 780-784 (2008) |
Background References 2 |
Nelson, D.R., Pharmacogenetics 14 (1), 1-18 (2004) |
Background References 3 |
Murray, G.I., FEBS Lett. 364 (1), 79-82 (1995) |
1st Image Caption |
Western blot analysis of CYP3A5 Antibody in 293 cell line lysates (35ug/lane) |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP3A5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
3rd Image Caption |
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.