Vergleich

BF Antibody

ArtNr PRS-62-850-400ul
Hersteller ProSci
Menge 400 ul
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, IHC-P
Specific against Human
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, Complement factor B Ba fragment, Complement factor B Bb fragment, CFB, BF, BFD
Lieferbar
Shipping
blue ice or RT
By Research Area
Immunology
Immunogen
This BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the Central region of human BF.
Applications
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight
86 kDa
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality
Polyclonal
Isotype
Rabbit Ig
Conjugate
Unconjugated
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration
batch dependent
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
CFB
Accession #
P00751
Protein Gi #
584908
Ncbi Gene Id #
629
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
CFB
Ncbi Official Full Name
Complement factor B
Ncbi Organism
Homo sapiens
Swissprot #
P00751
Background
BF is complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
Background References 1
Baatz, H., Klin Monatsbl Augenheilkd 226 (8), 654-658 (2009)
Background References 2
Goring, K., Am. J. Physiol., Cell Physiol. 296 (5), C1140-C1150 (2009)
1st Image Caption
Western blot analysis of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35ug/lane)
2nd Image Caption
BF Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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