ArtNr |
PRS-62-613-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Obesity, Signal Transduction |
Immunogen |
This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-277 amino acids from human PDK4. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
46 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PDK4 |
Accession # |
Q16654 |
Protein Gi # |
3183120 |
Ncbi Gene Id # |
5166 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PDK4 |
Ncbi Official Full Name |
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q16654 |
Background |
PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. |
Background References 1 |
Rosa, G., et al., Obes. Res. 11(2):176-182 (2003). Razeghi, P., et al., Cardiology 97(4):203-209 (2002). Rowles, J., et al., J. Biol. Chem. 271(37):22376-22382 (1996). Gudi, R., et al., J. Biol. Chem. 270(48):28989-28994 (1995). |
1st Image Caption |
Western blot analysis of PDK4 Antibody (E265) in CEM cell line lysates (35ug/lane) |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4-E265, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.