LAG3 Antibody

blue ice or RT

This LAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.

57 kDa

Polyclonal

Unconjugated

batch dependent

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

LAG3

251757512

Optimal dilutions for each application to be determined by the researcher.

Lymphocyte activation gene 3 protein

P18627

Smyth, D.J., et.al., BMC Med. Genet. 7, 20 (2006)

Overlay histogram showing Jurkat cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: mouse heart lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + mouse heart lysate Lysates/proteins at 20 ug per lane.