ArtNr |
PRS-62-352-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, FC, IF, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Hamster, Mouse, Rabbit, Rat, Chicken, Zebrafish |
Immunogen |
This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1. |
Applications |
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
50 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
EEF1A1 |
Accession # |
P68104 |
Protein Gi # |
55584035 |
Ncbi Gene Id # |
1915 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
EEF1A1 |
Ncbi Official Full Name |
Elongation factor 1-alpha 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P68104 |
Background |
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. |
Background References 1 |
Byun, H.O., Cancer Res. 69 (11), 4638-4647 (2009) |
1st Image Caption |
Western blot analysis of EEF1A1 Antibody in MCF-7 cell line lysates (35ug/lane) |
2nd Image Caption |
Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
3rd Image Caption |
Confocal immunofluorescent analysis of EEF1A1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
4th Image Caption |
EEF1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.