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EEF1A1 Antibody

ArtNr PRS-62-352-400ul
Hersteller ProSci
Menge 400 ul
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, FC, IF, IHC-P
Specific against Human
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7
Lieferbar
Shipping
blue ice or RT
By Research Area
Cancer
Homology
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Hamster, Mouse, Rabbit, Rat, Chicken, Zebrafish
Immunogen
This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1.
Applications
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight
50 kDa
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality
Polyclonal
Isotype
Rabbit Ig
Conjugate
Unconjugated
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration
batch dependent
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
EEF1A1
Accession #
P68104
Protein Gi #
55584035
Ncbi Gene Id #
1915
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
EEF1A1
Ncbi Official Full Name
Elongation factor 1-alpha 1
Ncbi Organism
Homo sapiens
Swissprot #
P68104
Background
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Background References 1
Byun, H.O., Cancer Res. 69 (11), 4638-4647 (2009)
1st Image Caption
Western blot analysis of EEF1A1 Antibody in MCF-7 cell line lysates (35ug/lane)
2nd Image Caption
Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
3rd Image Caption
Confocal immunofluorescent analysis of EEF1A1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
4th Image Caption
EEF1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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