Vergleich

KLF6 Antibody

ArtNr PRS-62-347-400ul
Hersteller ProSci
Menge 400 ul
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, FC, IF, IHC-P
Specific against Human, Mouse
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Krueppel-like factor 6, B-cell-derived protein 1, Core promoter element-binding protein, GC-rich sites-binding factor GBF, Proto-oncogene BCD1, Suppressor of tumorigenicity 12 protein, Transcription factor Zf9, KLF6, BCD1, COPEB, CPBP, ST12
Lieferbar
Shipping
blue ice or RT
By Research Area
Signal Transduction
Immunogen
This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6.
Applications
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Predicted Molecular Weight
32 kDa
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality
Polyclonal
Isotype
Rabbit Ig
Conjugate
Unconjugated
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration
batch dependent
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
KLF6
Accession #
Q99612
Protein Gi #
7387592
Ncbi Gene Id #
1316
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
KLF6
Ncbi Official Full Name
Krueppel-like factor 6
Ncbi Organism
Homo sapiens
Swissprot #
Q99612
Background
COPEB is a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor.
Background References 1
Sangodkar, J., Eur. J. Cancer 45 (4), 666-676 (2009) DiFeo, A., Drug Resist. Updat. 12 (1-2), 1-7 (2009)
1st Image Caption
Western blot analysis of KLF6 Antibody in mouse stomach tissue lysates (35ug/lane)
2nd Image Caption
Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3rd Image Caption
Flow cytometric analysis of widr cells using KLF6 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4th Image Caption
Fluorescent confocal image of Hela cell stained with KLF6 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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Lieferung vsl. bis 06.09.2024 

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