ArtNr |
PRS-62-347-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, FC, IF, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Krueppel-like factor 6, B-cell-derived protein 1, Core promoter element-binding protein, GC-rich sites-binding factor GBF, Proto-oncogene BCD1, Suppressor of tumorigenicity 12 protein, Transcription factor Zf9, KLF6, BCD1, COPEB, CPBP, ST12 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
32 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
KLF6 |
Accession # |
Q99612 |
Protein Gi # |
7387592 |
Ncbi Gene Id # |
1316 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
KLF6 |
Ncbi Official Full Name |
Krueppel-like factor 6 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q99612 |
Background |
COPEB is a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor. |
Background References 1 |
Sangodkar, J., Eur. J. Cancer 45 (4), 666-676 (2009) DiFeo, A., Drug Resist. Updat. 12 (1-2), 1-7 (2009) |
1st Image Caption |
Western blot analysis of KLF6 Antibody in mouse stomach tissue lysates (35ug/lane) |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
3rd Image Caption |
Flow cytometric analysis of widr cells using KLF6 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
4th Image Caption |
Fluorescent confocal image of Hela cell stained with KLF6 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.