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PARP1 Antibody

PARP1 Antibody

ArtNr	PRS-62-267-400ul
Hersteller	ProSci
Menge	400 ul
Kategorie	Neuroscience Molecular Targets for Neuroscience Neural Signaling Antibodies Flow Cytometry Western Blot Immunofluorescence Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, FC, IF
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL
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Shipping	blue ice or RT
By Research Area	Cell Cycle, Obesity
Homology	Predicted species reactivity based on immunogen sequence: Mouse
Immunogen	This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-214 amino acids from the N-terminal region of human PARP1.
Applications	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50
Predicted Molecular Weight	113 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PARP1
Accession #	P09874
Protein Gi #	130781
Ncbi Gene Id #	142
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PARP1
Ncbi Official Full Name	Poly [ADP-ribose] polymerase 1
Ncbi Organism	Homo sapiens
Swissprot #	P09874
Background	PARP1 is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Background References 1	Tao, Z., Biochemistry 47 (21), 5804-5813 (2008)
Background References 2	Gao, R., Mol. Cancer Ther.(5), 1246-1250 (2008)
Background References 3	Schreiber, V., EMBO J. 11 (9), 3263-3269 (1992)
1st Image Caption	Western blot analysis of PARP1 using rabbit polyclonal PARP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2).
2nd Image Caption	Western blot analysis in Hela cell line lysates (35ug/lane).
3rd Image Caption	Confocal immunofluorescent analysis of PARP1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
4th Image Caption	Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 400 ul

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Lieferung vsl. bis 06.09.2024

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