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DDAH1 Antibody

ArtNr PRS-61-587-400ul
Hersteller ProSci
Menge 400 ul
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, FC, IF, IHC-P
Specific against Human, Mouse
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias N(G),N(G)-dimethylarginine dimethylaminohydrolase 1, DDAH-1, Dimethylarginine dimethylaminohydrolase 1, DDAHI, Dimethylargininase-1, DDAH1, DDAH
Lieferbar
Shipping
blue ice or RT
By Research Area
Neuroscience
Homology
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen
This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1.
Applications
For IHC-P starting dilution is: 1:50~100

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Predicted Molecular Weight
31 kDa
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality
Polyclonal
Isotype
Rabbit Ig
Conjugate
Unconjugated
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration
batch dependent
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
DDAH1
Accession #
O94760
Ncbi Gene Id #
23576
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
DDAH1
Ncbi Official Full Name
N(G), N(G)-dimethylarginine dimethylaminohydrolase 1
Ncbi Organism
Homo sapiens
Swissprot #
O94760
Background
DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
Background References 1
Ellger, B., et.al., Endocrinology 149 (6), 3148-3157 (2008)
1st Image Caption
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
2nd Image Caption
Western blot analysis of DDAH1 Antibody in mouse kidney tissue lysates (35ug/lane)
3rd Image Caption
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4th Image Caption
Confocal immunofluorescent analysis of DDAH1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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