ArtNr |
PRS-61-517-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, FC, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP |
Lieferbar |
|
Shipping |
blue ice or RT |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine, Mouse |
Immunogen |
This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
28 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
U2AF1 |
Accession # |
Q01081 |
Protein Gi # |
267187 |
Ncbi Gene Id # |
102724594, 7307 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
U2AF1 |
Ncbi Official Full Name |
Splicing factor U2AF 35 kDa subunit |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q01081 |
Background |
U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. |
Background References 1 |
Rino, J., Mol. Cell. Biol. 28 (9), 3045-3057 (2008) Little, J.T., J. Biol. Chem. 283 (13), 8145-8152 (2008) Pacheco, T.R., Mol. Cell. Biol. 26 (21), 8183-8190 (2006) |
1st Image Caption |
Western blot analysis of anti- in Ramos cell line lysates (35ug/lane). |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
3rd Image Caption |
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
4th Image Caption |
Western blot analysis of U2AF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.