ArtNr |
PRS-61-473-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, FC, IF, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction, Growth Factor |
Immunogen |
This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
100 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
LGR5 |
Accession # |
O75473 |
Ncbi Gene Id # |
8549 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
LGR5 |
Ncbi Official Full Name |
Leucine-rich repeat-containing G-protein coupled receptor 5 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O75473 |
Background |
LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. |
Background References 1 |
Barker, N., Nature 449 (7165), 1003-1007 (2007) |
Background References 2 |
McClanahan, T., Cancer Biol. Ther.(4), 419-426 (2006) |
Background References 3 |
Yamamoto, Y., Hepatology 37 (3), 528-533 (2003) |
Background References 4 |
Hsu, S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000) |
1st Image Caption |
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li). |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
3rd Image Caption |
Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
4th Image Caption |
Confocal immunofluorescent analysis of LGR5/GPR49 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.