ArtNr |
PRS-59-680-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Retinoic acid receptor beta, RAR-beta, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2, RAR-epsilon, RARB, HAP, NR1B2 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Chicken, Mouse |
Immunogen |
This RARB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the Central region of human RARB. |
Applications |
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
50 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RARB |
Accession # |
P10826 |
Protein Gi # |
17380507 |
Ncbi Gene Id # |
5915 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RARB |
Ncbi Official Full Name |
Retinoic acid receptor beta |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P10826 |
Background |
This gene encodes retinoic acid receptor beta, a member ofthe thyroid-steroid hormone receptor superfamily of nucleartranscriptional regulators. This receptor localizes to thecytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellularsignalling in embryonic morphogenesis, cell growth anddifferentiation. It is thought that this protein limits growth ofmany cell types by regulating gene expression. The gene was firstidentified in a hepatocellular carcinoma where it flanks ahepatitis B virus integration site. The gene expresses at least twotranscript variants; one additional transcript has been described, but its full length nature has not been determined. [provided byRefSeq]. |
Background References 1 |
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
Background References 2 |
Miladi-Abdennadher, I., et al. Tumour Biol. 31(5):503-511(2010) |
Background References 3 |
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010) |
Background References 4 |
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) : |
1st Image Caption |
Western blot analysis in K562 cell line lysates (35ug/lane). |
2nd Image Caption |
Fluorescent confocal image of Hela cell stained with RARB Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.