ArtNr |
PRS-58-021-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, FC, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Cadherin-10, T2-cadherin, CDH10 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Chicken, Mouse |
Immunogen |
This CDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-523 amino acids from the C-terminal region of human CDH10. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
88 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CDH10 |
Accession # |
Q9Y6N8 |
Protein Gi # |
116241276 |
Ncbi Gene Id # |
1008 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CDH10 |
Ncbi Official Full Name |
Cadherin-10 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9Y6N8 |
Background |
CDH10 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. |
Background References 1 |
Kools, P., FEBS Lett. 452 (3), 328-334 (1999) |
1st Image Caption |
Western blot analysis of CDH10 Antibody in NCI-H460 cell line lysates (35ug/lane) |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH10 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
3rd Image Caption |
Flow cytometric analysis of NCI-H460 cells using Cadherin 10 (CDH10) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.