B-raf Antibody

Cancer, Cell Cycle

Anti-B-raf antibody (5777) was raised against a peptide corresponding to 18 amino acids near the center of human B-raf.

The immunogen is located within amino acids 330 - 380 of B-raf.

Cat. No. 1303 - Human Brain Tissue Lysate

Cat. No. 1224 – Daudi Cell Lysate

Predicted: 94kD

Observed: 94 kD

Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf has one isoform (712aa, 80kD). 5777 can detect in human B-raf and mouse isoform 1.

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 5777P – B-raf Peptide

None

P15056

673

BRAF

Homo sapiens

B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.

Figure 1 Western Blot Validation in Human Brain Tissue
Loading: 15 μ g of lysates per lane.Antibodies: B-raf 5777, (A:1 μ g/mL; B:2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Human Cell Lines
Loading: 15 μ g of lysates per lane.Antibodies: B-raf 5777, (0.5 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.