ArtNr |
PRS-5777-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Chicken |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle |
Homology |
Predicted species reactivity based on immunogen sequence: Chicken: (93%) |
Immunogen |
Anti-B-raf antibody (5777) was raised against a peptide corresponding to 18 amino acids near the center of human B-raf.
The immunogen is located within amino acids 330 - 380 of B-raf. |
Applications |
WB: 0.5-2 μ g/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1303 - Human Brain Tissue Lysate |
Positive Control 2 |
Cat. No. 1210 – 293 Cell Lysate |
Positive Control 3 |
Cat. No. 1224 – Daudi Cell Lysate |
Positive Control 4 |
Cat. No. 1204 – K562 Cell Lysate |
Predicted Molecular Weight |
Predicted: 94kD
Observed: 94 kD |
Validation |
Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. |
Isoforms |
Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf has one isoform (712aa, 80kD). 5777 can detect in human B-raf and mouse isoform 1. |
Purification |
B-raf Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgY |
Conjugate |
Unconjugated |
Buffer |
B-raf Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
B-raf antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5777P – B-raf Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
BRAF |
Accession # |
P15056 |
Protein Gi # |
50403720 |
Ncbi Gene Id # |
673 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
BRAF |
Ncbi Official Full Name |
v-raf murine sarcoma viral oncogene homolog B1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P15056 |
Background |
B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance. |
Background References 1 |
McCubrey et al. Biophys. Acta 2007; 1773:1263-84. |
Background References 2 |
Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8. |
Background References 3 |
Sarkozy et al. Hum. Mutat.2009; 30:695-702. |
1st Image Caption |
Figure 1 Western Blot Validation in Human Brain Tissue Loading: 15 μ g of lysates per lane.Antibodies: B-raf 5777, (A:1 μ g/mL; B:2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution. |
2nd Image Caption |
Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: B-raf 5777, (0.5 μ g/mL), B-raf 7379, (0.5 μ g/mL) and GAPDH (0.02 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken (for 5777) and goat anti-rabbit (for 7379) IgG HRP conjugate at 1:10000 dilution. |
3rd Image Caption |
Figure 3 Western Blot Validation in Human Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: B-raf 5777, (0.5 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.