ArtNr |
PRS-5761-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RBBP8 Antibody: RIM, COM1, CTIP, JWDS, SAE2, SCKL2, DNA endonuclease RBBP8, CtBP-interacting protein, CtIP |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle |
Immunogen |
RBBP8 antibody was raised against a 14 amino acid synthetic peptide near the center of human RBBP8.
The immunogen is located within amino acids 430 - 480 of RBBP8. |
Applications |
RBBP8 antibody can be used for detection of RBBP8 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1406 - Mouse Spleen Tissue Lysate |
Purification |
RBBP8 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
RBBP8 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
RBBP8 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5761P – RBBP8 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RBBP8 |
Accession # |
NP_976037 |
Protein Gi # |
42718017 |
Ncbi Gene Id # |
5932 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RBBP8 |
Ncbi Official Full Name |
retinoblastoma binding protein 8 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q99708 |
Background |
RBBP8 Antibody: RBBP8, also known as CtBP (carboxy-terminal binding protein) interacting protein (CTIP), was characterized for its role in transcription as a cofactor for the transcriptional repressor CtBP, and also as a binding partner for other proteins including the cell cycle regulators retinoblastoma protein (Rb) and breast cancer 1 (BRCA1). It is ubiquitously expressed and localizes to the nucleus. RBBP8 is thought to modulate the functions in cell proliferation, transcriptional regulation and DNA repair. RBBP8 also plays a central role in the cell cycle checkpoint response to DNA double-stranded breaks (DSBs), with new evidence demonstrating that it controls the choice of DSB repair pathway. |
Background References 1 |
Yu X, Wu LC, Bowcock AM, et al. The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression. J. Biol. Chem.1998; 273:25388-92. |
Background References 2 |
Liu F and Lee WH. CtIP activates its own and cyclin D1 promoters via the E2F/RB pathway during G1/S progression. Mol. Cell Biol.2006; 26:3124-34. |
Background References 3 |
Sterner JM, Dew-Knight S, Musahl C, et al. Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. Mol. Cell Biol.1998; 18:2748-57. |
Background References 4 |
Wu G and Lee WH. CtIP, a multivalent adaptor connecting transcriptional regulation, checkpoint control and tumor suppression. Cell Cycle2006; 5:1592-6. |
1st Image Caption |
Western blot analysis of RBBP8 in mouse spleen tissue lysate with RBBP8 antibody at (A) 1 and (B) 2 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of RBBP8 in rat spleen tissue with RBBP8 antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of RBBP8 in rat spleen tissue with RBBP8 antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.