ArtNr |
PRS-57-727-400ul |
Hersteller |
ProSci
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Histamine N-methyltransferase, HMT, HNMT |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Immunogen |
This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT. |
Applications |
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
33 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HNMT |
Accession # |
P50135 |
Protein Gi # |
1708272 |
Ncbi Gene Id # |
3176 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HNMT |
Ncbi Official Full Name |
Histamine N-methyltransferase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P50135 |
Background |
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. |
Background References 1 |
Aksoy, S., et al., Biochem. Biophys. Res. Commun. 219(2):548-554 (1996). |
1st Image Caption |
Confocal immunofluorescent analysis of HNMT Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
2nd Image Caption |
Western blot analysis of HNMT Antibody in Hela cell line lysates (35ug/lane). HNMT was detected using the purified polyclonal antibody. |
3rd Image Caption |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.