DCLK1 Antibody

blue ice or RT

DCLK1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK1.

The immunogen is located within the last 50 amino acids of DCLK1.

Cat. No. 1303 - Human Brain Tissue Lysate

Predicted: 90 kDa

Observed: 85 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 5633P – DCLK1 Peptide

None

NP_004725

9201

DCLK1

Homo sapiens

DCLK1 Antibody: DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.

Ohmae S, Takemoto-Kimura S, Okamura M, et al. Molecular identification and characterization of a family of kinases with homology to Ca2+/calmodulin-dependent protein kinases I/IV. J. Biol. Chem.2006; 281:20427-39.

Reiner O and Coquelle FM. Missense mutations resulting in type 1 lissencephaly. Cell Mol. Life Sci.2005; 62:425-34.

Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 μ g/mL.

Immunohistochemistry of DCLK1 in mouse brain tissue with DCLK1 Antibody at 5 μ g/mL.