APP Antibody

Neuroscience

APP antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APP.

The immunogen is located within amino acids 180 - 230 of APP.

Cat. No. 1463 - Rat Brain Tissue Lysate

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 5143P – APP Peptide

None

CAA30050

351

APP

Homo sapiens

APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. Recently, Death Receptor 6 (DR6) was found to interact with an amino-terminal fragment of the Beta-amyloid protein (N-APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning during development, suggesting that these two proteins are involved in neural development and may possibly play a role in Alzheimer's disease.

Selkoe DJ. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu Rev Cell Biol1994; 10:373-403.

Western blot analysis of APP in rat brain tissue lysate with APP antibody at (A) 1 and (B) 2 μ g/mL.

Immunofluorescence of APP in mouse brain tissue with APP antibody at 20 μ g/mL.

Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 μ g/mL.

Green: APP antibody (5143)
Red: Phylloidin staining
Blue: DAPI staining