ArtNr |
PRS-5081-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PCDH18 Antibody: PCDH68L, KIAA1562, Protocadherin-18 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine: (95%) |
Immunogen |
PCDH18 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH18.
The immunogen is located within amino acids 50 - 100 of PCDH18. |
Applications |
PCDH18 antibody can be used for detection of PCDH18 by Western blot at 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Specificity |
At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Purification |
PCDH18 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PCDH18 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
PCDH18 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5081P – PCDH18 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PCDH18 |
Accession # |
NP_061908 |
Protein Gi # |
14589929 |
Ncbi Gene Id # |
54510 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PCDH18 |
Ncbi Official Full Name |
protocadherin 18 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9HCL0 |
Background |
PCDH18 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 18 (PCDH18) was initially identified through screening of human and mouse ESTs using conserved cytoplasmic domain motifs. Tissue screening revealed PCDH18 broad expression in tissues such as brain, liver, heart, kidney, lung, and trachea. PCDH18 expression is also temporally and spatially regulated in the mouse embryonic brain and interacts with Disabled-1, an intracellular adapter protein involved in neuronal migration and cell positioning during mammalian brain development. PCDH18 was also found to be expressed in the developing zebrafish neural tube and central nervous system, lending support to the hypothesis that PCDH18 may play a role during brain development. |
Background References 1 |
Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol.2002; 14:557-62. |
Background References 2 |
Wolverton T and Lalande M. Identification and characterization of three members of a novel subclass of protocadherins. Genomics2001; 76:66-72. |
Background References 3 |
Homayouni R, Rice DS, and Curran T. Disabled-1 interacts with a novel developmentally regulated protocadherin. Biochem. Biophys. Res. Commun.2001; 289:539-47. |
Background References 4 |
Kubota F, Murakami T, Tajika Y, et al. Expression of protocadherin 18 in the CNS and pharyngeal arches of zebrafish embryos. Int. J. Dev. Biol.2008; 52:397-405. |
1st Image Caption |
Western blot analysis of PCDH18 in HepG2 cell lysate with PCDH18 antibody at 1 μ g/mL in (A) the absence and (B) the presence of blocking peptide. |
2nd Image Caption |
Immunohistochemistry of PCDH18 in mouse brain tissue with PCDH18 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of PCDH18 in Mouse Brain cells with PCDH18 antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.