ArtNr |
PRS-50-210-0.1mL |
Hersteller |
ProSci
|
Menge |
0.1 mL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IHC |
Specific against |
Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Immunogen |
Fusion protein from N-terminal region of the a3-subunit of rat GABAA receptor. |
Applications |
The antibody has been directly tested for reactivity in Western blots with rat and mouse tissue. |
Predicted Molecular Weight |
51 |
Purification |
Affinity Purified |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Storage Conditions |
GABAA Receptor antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
Gabra3 |
Accession # |
P20236 |
Protein Gi # |
120761 |
Ncbi Gene Id # |
24947 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
Gabra3 |
Ncbi Official Full Name |
gamma-aminobutyric acid (GABA) A receptor, alpha 3 |
Ncbi Organism |
Rattus norvegicus |
Swissprot # |
P20236 |
Background |
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). |
Background References 1 |
McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor α 1-subtype. Nature Neurosci 3:587-592. |
Background References 2 |
Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. |
Background References 3 |
Ogris W, Pö ltl A, Hauer B, Ernst M, Oberto A, Wulff P, Hö ger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor γ 2-subunit. Biochem Pharmacol 68:1621-1629. |
1st Image Caption |
Western blot of rat brain lysates from wild type (Control) and alpha3-knockout (alpha3-K/O) animals showing specific immunolabeling of the ~51k alpha3-subunit of the GABAA receptor. The labeling was absent from a lysate prepared from alpha3-knockout animals. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.