Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Format |
Liquid |
Applikationen |
WB, ELISA |
Menge |
0.02 mg |
Host |
Rabbit |
ArtNr |
PRS-4817-0.02mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Alias |
ZBRK1 Antibody: ZFQR, ZBRK1, Zinc finger protein 350, KRAB zinc finger protein ZFQR |
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Immunogen |
ZBRK1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human ZBRK1.
The immunogen is located within amino acids 410 - 460 of ZBRK1. |
Applications |
ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 μ g/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1288 - A20 Cell Lysate |
Purification |
ZBRK1 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
ZBRK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
ZBRK1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4817P – ZBRK1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ZNF350 |
Accession # |
NP_067645 |
Protein Gi # |
59710107 |
Ncbi Gene Id # |
59348 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ZNF350 |
Ncbi Official Full Name |
zinc finger protein 350 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9GZX5 |
Background |
ZBRK1 Antibody: ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions. |
Background References 1 |
Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell2000; 6:757-68. |
Background References 2 |
Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol.2001; 21:3609-15. |
Background References 3 |
Tan W, Kim S, and Boyer TG. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated Box-Zinc finger protein ZBRK1. J. Biol. Chem.2004; 279:55153-60. |
1st Image Caption |
Western blot analysis of ZBRK1 in A-20 lysate with ZBRK1 antibody at 1 μ g/mL in (A) the absence and (B) the presence of blocking peptide. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.