ArtNr |
PRS-4771-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
GPVI Antibody: GPIV, GPVI, BDPLT11, Platelet glycoprotein VI, Glycoprotein 6 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Immunogen |
GPVI antibody was raised against a 18 amino acid synthetic peptide near the center of the human GPVI.
The immunogen is located within amino acids 130 - 180 of GPVI. |
Applications |
GPVI antibody can be used for detection of GPVI by Western blot at 1 μ g/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1205 – Jurkat Cell Lysate |
Predicted Molecular Weight |
Predicted: 37, 68 kDa
Observed: 55 kDa |
Purification |
GPVI Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
GPVI Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
GPVI antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4771P – GPVI Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
GP6 |
Accession # |
NP_057447 |
Protein Gi # |
143770755 |
Ncbi Gene Id # |
51206 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
GP6 |
Ncbi Official Full Name |
glycoprotein VI (platelet) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9HCN6 |
Background |
GPVI Antibody: Glycoprotein VI (GP6) is a 58kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. It is uniquely expressed by cells of the megakaryocytic/platelet lineage, and is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. Glycoprotein VI plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. GPVI deficiency can result in bleeding disorders. Further study should reveal the extent of GPVI involvement in thrombotic disease and allow the development of alternative anti-thrombotic compounds. |
Background References 1 |
Jarvis GE, Atkinson BT, Snell DC, et al. Distinct roles of GPVI and integrin alpha(2) beta(1) in platelet shape change and aggregation induced by different collagens. Br. J. Pharmacol.2002; 137:107-17. |
Background References 2 |
Inoue O, Suzuki-Inoue K, Dean WL, et al. Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2. J. Cell Biol.2003; 160:769-80. |
Background References 3 |
Clemetson JM, Polgar J, Magnenat E, et al. The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to Fcα R and the natural killer receptors. J Biol. Chem.1999; 274:29019-24. |
Background References 4 |
Jandrot-Perrus M, Busfield S, Lagrue AH, et al. Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily. Blood2000; 96:1798-807. |
1st Image Caption |
Western blot analysis of GPVI expression in Jurkat cell lysate with GPVI antibody at 1 μ g/ml in (A) the absence and (B) the presence of blocking peptide. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.