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SH2D1A Antibody

SH2D1A Antibody

ArtNr	PRS-46-365-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Metabolism Glucose Homeostasis Antibodies Immunoprecipitation Western Blot ELISA Primary Antibodies
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IP, ELISA
Specific against	Human
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SH2D1A, SAP, SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome), HGNC:10820, DSHP, EBVS, IMD5, LYP, MTCP1, XLP, XLPD , SH2 domain protein 1A, SLAM (signaling lymphocyte activation molecule) associated protein, RP5-1052M9.3, FLJ18687,
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Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen	The immunogen for this antibody is: C-HKRYFRKIKN
Applications	Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 16kDa band observed in Human Thymus lysates (calculated MW of 14.2xkDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 16kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
Specificity	.
Positive Control 1	Cat. No. 1314 - Human Thymus Tissue Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 46-365P - SH2D1A Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	SH2D1A
Accession #	NP_002342.1, NP_001108409.1
Protein Gi #	4506923
Ncbi Gene Id #	4068
Ncbi Official Symbol	SH2D1A
Ncbi Official Full Name	SH2 domain containing 1A
Ncbi Organism	Homo sapiens
Swissprot #	O60880
Background References 1	Mikhalap SV, Shlapatska LM, Yurchenko OV, Yurchenko MY, Berdova GG, Nichols KE, Clark EA, Sidorenko SP. The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells. Blood. 2004 Dec 15; 104(13):4063-70. Epub 2004 Aug 17.
1st Image Caption	46-365 staining (0.3ug/ml) of Human Thymus lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
2nd Image Caption	HEK293 overexpressing SH2D1A and probed with 46-365 (mock transfection in first lane).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.1 mg

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