ArtNr |
PRS-46-365-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IP, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
SH2D1A, SAP, SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome), HGNC:10820, DSHP, EBVS, IMD5, LYP, MTCP1, XLP, XLPD , SH2 domain protein 1A, SLAM (signaling lymphocyte activation molecule) associated protein, RP5-1052M9.3, FLJ18687, |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Rat |
Immunogen |
The immunogen for this antibody is: C-HKRYFRKIKN |
Applications |
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 16kDa band observed in Human Thymus lysates (calculated MW of 14.2xkDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 16kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer). |
Specificity |
. |
Positive Control 1 |
Cat. No. 1314 - Human Thymus Tissue Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 46-365P - SH2D1A Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SH2D1A |
Accession # |
NP_002342.1, NP_001108409.1 |
Protein Gi # |
4506923 |
Ncbi Gene Id # |
4068 |
Ncbi Official Symbol |
SH2D1A |
Ncbi Official Full Name |
SH2 domain containing 1A |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O60880 |
Background References 1 |
Mikhalap SV, Shlapatska LM, Yurchenko OV, Yurchenko MY, Berdova GG, Nichols KE, Clark EA, Sidorenko SP. The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells. Blood. 2004 Dec 15; 104(13):4063-70. Epub 2004 Aug 17. |
1st Image Caption |
46-365 staining (0.3ug/ml) of Human Thymus lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
2nd Image Caption |
HEK293 overexpressing SH2D1A and probed with 46-365 (mock transfection in first lane). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.