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RNF23 Antibody

RNF23 Antibody

ArtNr	PRS-46-307-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Oncology Antibodies ELISA Primary Antibodies By Organ Testicular & Prostate Cancer
Typ	Antibody Primary
Format	Liquid
Applikationen	IHC, ELISA
Specific against	Human
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	TRIM39, TRIM39B, TFP, RNF23, MGC32984, tripartite motif-containing 39, ring finger protein 23, testis-abundant finger protein, DAAP-385L22.4, OTTHUMP00000029075, OTTHUMP00000029076
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Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen	The immunogen for this antibody is: C-NAAPLTIRPPTDWE
Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 49kDa band in Human Testis and Rat Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_NP_742013.1 and of 59.7kDa according to NP_067076.2. The 49kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75ug/ml.
Specificity	This antibody is expected to recognise both reported isoforms (NP_067076.2 and NP_742013.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 46-307P - RNF23 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	TRIM39
Accession #	NP_067076.2, NP_742013.1
Protein Gi #	25777696
Ncbi Gene Id #	56658
Ncbi Official Symbol	TRIM39
Ncbi Official Full Name	tripartite motif containing 39
Ncbi Organism	Homo sapiens
Swissprot #	Q9HCM9
Background References 1	Orimo A, Yamagishi T, Tominaga N, Yamauchi Y, Hishinuma T, Okada K, Suzuki M, Sato M, Nogi Y, Suzuki H, Inoue S, Yoshimura K, Shimizu Y, Muramatsu M. Molecular cloning of testis-abundant finger Protein/Ring finger protein 23 (RNF23), a novel RING-B box-coiled coil-B30.2 protein on the class I region of the human MHC. Biochem Biophys Res Commun. 2000 Sep 16; 276(1):45-51.
1st Image Caption	46-307 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Menge: 0.1 mg

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