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Neuro-d4 Antibody

Neuro-d4 Antibody

ArtNr	PRS-45-954-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Oncology Antibodies Western Blot ELISA Primary Antibodies By Organ Brain Cancer
Typ	Antibody Primary
Format	Liquid
Applikationen	IF, ELISA
Specific against	Human
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	DPF1, NEUD4, neuro-d4, D4, zinc and double PHD fingers family 1, Neuro-d4 (rat) homolog, D4, zinc and double PHD fingers, family 1, MGC150428, MGC150429, BAF45b, OTTHUMP00000211615, neuro-d4 homolog
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Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen	The immunogen for this antibody is: C-HLKEKASAYITLT
Applications	Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments showed a band at approx 38-40kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1ug/ml antibody staining (calculated MW of 37.9kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml.
Specificity	This antibody is expected to recognize all three reported isoforms (NP_001128627.1; NP_004638.2; NP_001128628.1).
Positive Control 1	Cat. No. 1362 - Human Cerebellum, Left Lysate Cat. No. 1363 - Human Cerebellum, Right Lysate
Positive Control 2	Cat. No. XBL-10131 - Human Amygdala Tissue Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-954P - Neuro-d4 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	DPF1
Accession #	NP_001128627.1, NP_004638.2, NP_001128628.1
Protein Gi #	4758798
Ncbi Gene Id #	8193
Ncbi Official Symbol	DPF1
Ncbi Official Full Name	D4, zinc and double PHD fingers family 1
Ncbi Organism	Homo sapiens
Swissprot #	Q92782
Background References 1	Chestkov AV, Baka ID, Kost MV, Georgiev GP, Buchman VL. The d4 gene family in the human genome. Genomics. 1996 Aug 15; 36(1):174-7.
1st Image Caption	45-954 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The
2nd Image Caption	45-954 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The

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Menge: 0.1 mg

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