ArtNr |
PRS-45-942-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
IHC, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
NEIL1, NEH1, nei endonuclease VIII-like 1 (E. coli), NEI1, FLJ22402, endonuclease VIII, FPG1, hFPG1, nei endonuclease VIII-like 1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Pig, Cow |
Immunogen |
The immunogen for this antibody is: C-DIPSLEPEGTSAS |
Applications |
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10ug/ml. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1309 - Human Placenta Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-942P - NEIL1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NEIL1 |
Accession # |
NP_078884.2 |
Protein Gi # |
13375817 |
Ncbi Gene Id # |
79661 |
Ncbi Official Symbol |
NEIL1 |
Ncbi Official Full Name |
nei endonuclease VIII-like 1 (E. coli) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q96FI4 |
Background References 1 |
Hazra TK, Izumi T, Boldogh I, Imhoff B, Kow YW, Jaruga P, Dizdaroglu M, Mitra S. Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA. Proc Natl Acad Sci USA. 2002 Mar 19; 99(6):3523-8. |
1st Image Caption |
45-942 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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