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JUP Antibody

JUP Antibody

ArtNr	PRS-45-076-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Antibodies Western Blot ELISA Primary Antibodies
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IHC, ELISA
Specific against	Human, Mouse, Rat
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa
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Shipping	blue ice or RT
Homology	Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen	The immunogen for this antibody is: C-GLRPPYPTADHMLA
Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificity	This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
Positive Control 1	Cat. No. 1202 - A431 Cell Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-076P - JUP Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	JUP
Accession #	NP_002221.1, NP_068831.1
Protein Gi #	4504811
Ncbi Gene Id #	3728
Ncbi Official Symbol	JUP
Ncbi Official Full Name	junction plakoglobin
Ncbi Organism	Homo sapiens
Swissprot #	P14923
Background References 1	Knudsen KA, Wheelock MJ. Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin. J Cell Biol. 1992 Aug; 118(3):671-9.
1st Image Caption	45-076 (0.3ug/ml) staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption	45-076 staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
3rd Image Caption	45-076 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.1 mg

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