ArtNr |
PRS-45-076-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IHC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa |
Lieferbar |
|
Shipping |
blue ice or RT |
Homology |
Expected Species Reactivity based on sequence homology: Dog, Pig |
Immunogen |
The immunogen for this antibody is: C-GLRPPYPTADHMLA |
Applications |
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
Specificity |
This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1. |
Positive Control 1 |
Cat. No. 1202 - A431 Cell Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-076P - JUP Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
JUP |
Accession # |
NP_002221.1, NP_068831.1 |
Protein Gi # |
4504811 |
Ncbi Gene Id # |
3728 |
Ncbi Official Symbol |
JUP |
Ncbi Official Full Name |
junction plakoglobin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P14923 |
Background References 1 |
Knudsen KA, Wheelock MJ. Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin. J Cell Biol. 1992 Aug; 118(3):671-9. |
1st Image Caption |
45-076 (0.3ug/ml) staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
2nd Image Caption |
45-076 staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence. |
3rd Image Caption |
45-076 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.