Nhe-1 Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Rabbit: (95%), Pig: (95%), Bovine: (95%)

Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Cat. No. 1465 - Rat Kidney Tissue Lysate

Predicted: 52, 90 kDa

Observed: 50, 90 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 4377P – Nhe-1 Peptide

None

P19634

6548

SLC9A1

Homo sapiens

Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension.

Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell. 2000; 6:1425-36.

Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 μ g/mL.

Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20 μ g/mL.