ArtNr |
PRS-4377-0.02mg |
Hersteller |
ProSci
|
Menge |
0.02 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Nhe-1 Antibody: APNH, NHE1, NHE-1, APNH1, APNH |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Homology |
Predicted species reactivity based on immunogen sequence: Rabbit: (95%), Pig: (95%), Bovine: (95%) |
Immunogen |
Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1.
The immunogen is located within the last 50 amino acids of Nhe-1. |
Applications |
Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
At least three isoforms of Nhe-1 are known to exist. |
Positive Control 1 |
Cat. No. 1465 - Rat Kidney Tissue Lysate |
Positive Control 2 |
Cat. No. 10-301 - Human Brain Tissue Slide |
Predicted Molecular Weight |
Predicted: 52, 90 kDa
Observed: 50, 90 kDa |
Purification |
Nhe-1 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
Nhe-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4377P – Nhe-1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SLC9A1 |
Accession # |
P19634 |
Protein Gi # |
127809 |
Ncbi Gene Id # |
6548 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SLC9A1 |
Ncbi Official Full Name |
solute carrier family 9 (sodium/hydrogen exchanger), member 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P19634 |
Background |
Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. |
Background References 1 |
Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand. 1987; 76:545-7. |
Background References 2 |
Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell. 2000; 6:1425-36. |
Background References 3 |
Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+)/H(+) exchange inhibition. Hypertension 2003; 41:373-7. |
1st Image Caption |
Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 μ g/mL. |
2nd Image Caption |
Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.